Quantitative Analysis of Plant Hormones

Targeted metabolomics

Metabolic Flux Analysis

Quantitative Analysis of Energy Metabolism

Quantitative Analysis of Short-Chain Fatty Acids

Quantitative Analysis of Fatty Acids

Quantitative Analysis of Bile Acids

uantitative Analysis of Trimethylamine Oxide and Related Metabolites

Quantitative Analysis of Amino Acids

Quantitative Analysis of Neurotransmitters

Quantitative Analysis of Organic Acids

Quantitative Analysis of Flavonoids

Quantitative Analysis of Carbohydrates

Quantitative Analysis of Plant Hormones

Quantitative Analysis of Carotenoids

Quantitative Analysis of Tannins

Quantitative Analysis of Phenolic Acids

Quantitative Analysis of Anthocyanins

Quantitative Analysis of Vitamins

Quantitative Analysis of Arachidonic Acids

Quantitative Analysis of Plant Hormones

Plant hormones are also called plant natural hormones or plant endogenous hormones. Organic compounds produced by plants in minute amounts that regulate (promote or inhibit) their own physiological processes.

Application Fields

Fruit and vegetable storage

Agronomic traits

Seed dormancy and germination

Gene expression regulation

Plant growth and development regulation

Biological Stress (Pests and Diseases)

Abiotic stress

Sample Requirements

Case Analysis

Distinct Metabolism of N-glucosides of Isopentenyladenine and Trans-zeatin Determines Cytokinin Metabolic Spectrum in Arabidopsis

Journal: New Phytologist       Impact factor: 8.512 Published date: 2020       Published by: Czech Academy of Sciences

Research Background

The diversity of cytokinin (CK) metabolites suggests their interconversions are the predominant regulatory mechanism of CK action. Nevertheless, little is known about their directionality and kinetics in planta.

Research Method

CK metabolite levels were measured in 2-wk-old Arabidopsis thaliana plants at several time points up to 100 min following exogenous application of selected CKs. The data were then evaluated qualitatively and by mathematical modeling. Apart from elevated levels of trans-zeatin (tZ) metabolites upon application of N6-(Δ2-isopentenyl)adenine (iP), we observed no conversions between the individual CK-types – iP, tZ, dihydrozeatin (DHZ) and cis-zeatin (cZ). In particular, there was no sign of isomerization between tZ and cZ families. Also, no increase of DHZ-type CKs was observed after application of tZ, suggesting low baseline activity of zeatin reductase. Among N-glucosides, those of iP were not converted back to iP while tZ N-glucosides were cleaved to tZ bases, thus affecting the whole metabolic spectrum.

Research Result

We present the first large-scale study of short-term CK metabolism kinetics and show that tZ N7- and N9-glucosides are metabolized in vivo.


Studies show that N-glycosylation inactivates CKs irreversibly .


Hoš ek Petr,Hoyerová Klára,Kiran Nagavalli S et al. Distinct metabolism of N-glucosides of isopentenyladenine and trans-zeatin determines cytokinin metabolic spectrum in Arabidopsis.[J] .New Phytol., 2020, 225: 2423-2438

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