Quantitative Analysis of Plant Hormones

Plant hormones are also called plant natural hormones or plant endogenous hormones. Organic compounds produced by plants in minute amounts that regulate (promote or inhibit) their own physiological processes.

Sample Requirements

• Solid 2g
• Sample type: plant tissue
• Recommended to repeat more than 6 times

• Liquid/solid samples, dry ice shipping; large pieces need to be cut into 1cm pieces

Case Analysis

Journal: New Phytologist       Impact factor: 8.512 Published date: 2020       Published by: Czech Academy of Sciences

Research Background

The diversity of cytokinin (CK) metabolites suggests their interconversions are the predominant regulatory mechanism of CK action. Nevertheless, little is known about their directionality and kinetics in planta.

Research Method

CK metabolite levels were measured in 2-wk-old Arabidopsis thaliana plants at several time points up to 100 min following exogenous application of selected CKs. The data were then evaluated qualitatively and by mathematical modeling. Apart from elevated levels of trans-zeatin (tZ) metabolites upon application of N6-(Δ2-isopentenyl)adenine (iP), we observed no conversions between the individual CK-types – iP, tZ, dihydrozeatin (DHZ) and cis-zeatin (cZ). In particular, there was no sign of isomerization between tZ and cZ families. Also, no increase of DHZ-type CKs was observed after application of tZ, suggesting low baseline activity of zeatin reductase. Among N-glucosides, those of iP were not converted back to iP while tZ N-glucosides were cleaved to tZ bases, thus affecting the whole metabolic spectrum.

Research Result

We present the first large-scale study of short-term CK metabolism kinetics and show that tZ N7- and N9-glucosides are metabolized in vivo.

Diagram of cytokinin (CK) transformation in Arabidopsis thaliana

After iP, tZ, DHZ and Cz treatment, the mutual transformation of cytokinin (CK) group was redundant

In 14d old Arabidopsis seedlings, n-glycosides of iP and tZ were transformed into cytokinin bases at different rates

Conclusion

Studies show that N-glycosylation inactivates CKs irreversibly .

Reference

Hoš ek Petr,Hoyerová Klára,Kiran Nagavalli S et al. Distinct metabolism of N-glucosides of isopentenyladenine and trans-zeatin determines cytokinin metabolic spectrum in Arabidopsis.[J] .New Phytol., 2020, 225: 2423-2438