Ionomics

Ionomics is an emerging discipline that studies the composition, distribution and accumulation of elements in the body, as well as the changes and mechanisms of these elements in random body physiological conditions, biological and abiotic stimuli, developmental stages, environment and genetics. All metals, metalloids and non-metals. Ionic elements are widely involved in various important life activities and perform important physiological functions.

Application Fields

Application of phytochemical element detection

Environmental assessment and prediction, screening and application of hyperaccumulators in the field of environmental remediation, study of heavy metal detoxification mechanism, contaminated soil remediation and study of heavy metal exclusion mechanism.

Application direction of heavy metal content detection

Anthocyanins regulate the absorption and transport of heavy metals by plants scavenging free radicals, promoting and activating antioxidant systems, chelating with heavy metals, compartmentalizing isolation, anthocyanin structure/regulating gene expression, etc., thereby alleviating the toxicity of heavy metals to plants.

Sample Requirements

Cells ≥ 10⁷
Recommended to repeat more than 6 times
Liquid 500μl
Tissue and other solid samples 200mg

Sample type: animal and plant tissues, cells, blood; plant tissues, fruits, seeds; soil, etc.

samples, dry ice shipping; large pieces need to be cut into pieces of about 1cm

Case Analysis

Genetic Basis of Rice Ionomic Variation Revealed by Genome-Wide Association Studies

Journal: The Plant Cell Impact factor: 8.631 Published date: October, 2018 Published by: Huazhong Agricultural University, National Plant Genetic Research Center

Research Background

Rice (Oryza sativa) is an important dietary source of both essential micronutrients and toxic trace elements for humans. The genetic basis of mineral composition (ion) variation in rice remains largely unknown.

Research Design

Using genome-wide association studies (GWAS), the research team measured the content of 17 mineral elements in straw and grains using 529 samples of rice from fields in Hubei and Hunan over several years, the genetic basis of rice ion group natural variation was analyzed by association analysis with single base sequence polymorphism information of rice genome.

Research Result

32 loci were detected repeatedly in different sites, and 40 loci were detected repeatedly in different field trials at the same site. The candidate genes of 42 loci, including OSHKT1; 5, OSMOT1; 1 and Ghd7, were predicted, this study provided available genetic loci and theoretical basis for genetic improvement of rice nutrition quality.

Figure 1 Based on the cluster analysis of rice natural population, the cluster analysis of the main components including field conditions, sub-groups and tissues was carried out by using the concentration of each element

Figure 2 Distribution of 72 common loci scanned repeatedly on 12 chromosomes according to physical distance

Figure 3 Analysis of Na concentration in different Os-HKT1:5 haplotypes. The letters on the color background are one-letter codes for the corresponding amino acid residues. The same and different amino acid residues in HAP2 are shown in red and blue

Figure 4 Based on near-isogenic lines and transgenic plants, the role of Ghd7 in nitrogen accumulation in rice was analyzed

Conclusion

A comprehensive study on the genetic structure of rice ion variation will help to reveal the regulatory mechanism of mineral nutrition and microelement composition in plants.

Reference

Yang M , Lu K , Zhao F J , et al. Genetic basis of rice ionomic variation revealed by Genome-wide association studies[J]. The Plant Cell, 2018, 30