Transcriptomics

Transcriptomics is the study of all RNAs transcribed by a specific cell, tissue or individual at a specific time and state. Transcriptome sequencing uses the high-throughput sequencing methods to study specific cells, tissues or individuals at a specific time and state. All mRNAs transcribed under different functional states are used to reveal the differences in gene expression and structure under different functional states, and to clarify the molecular mechanism.

Application Fields

Medical research

Disease marker screening, disease diagnosis and classification, disease recurrence diagnosis, etiology and pathological mechanism exploration, clinical efficacy evaluation, pharmacotoxicological evaluation

Life science research

Research on abiotic environment relationships,mPlants and microbes, Application in phenotypic identification, Metabolic pathway and functional genomics research, Application in medicinal plant research

Product Type

CircRNA sequencing
LncRNA sequencing
Small RNA sequencing
Whole transcriptome sequencing

Eukaryotic transcriptome sequencing
Prokaryotic transcriptome sequencing

Sample Requirements

Animal and plant tissue ≥ 0.1g
Cells ≥ 10⁵
Fungus ≥ 0.2g
Bacteria ≥ 0.2g
Store in liquid nitrogen or -80°C
Dry ice shipping

Case Analysis

Turing miRNA into Infnite Coordination Supermolecule: A General and Enabling Nanoengineering Strategy for Resurrecting Nuclear Acid Therapeutics

Journal of Nanobiotechnology Impact factor: 10.435 Published date: 2022 Published by: The Second Affiliated Hospital of Xi'an Jiaotong University

Research Background

Nucleic acids exist in cell nucleus and mitochondria, they are important compounds in organism, they have important functions of storing and transmitting genetic information, and they are the basis material of genes. The clinical application of therapeutic nucleic acid, especially in the treatment of tumor, has broad prospects for development. However, due to its inherent weaknesses, poor system stability, fast clearance, low membrane permeability and the lack of targeting capabilities. Engineering small nucleic acids into carrier-free nanomedicines with structural stability and disease targeting may be a feasible way to overcome the nucleic acid medicine barrier. The second affiliated hospital of the Xi'an Jiaotong University is committed to this area of research, and has a number of research results in this field.

Technical Route

Research Result

Figure 1 Preparation of lacsRNA and its therapeutic mechanism

Figure 2 Physicochemical and pharmacological properties of lacsRNA

Figure 3 Biosafety evaluation of lacsRNA in vivo

Figure 4 targeting Bcl9 inhibits the Wnt pathway

Figure 5 LacsRNA inhibits patient-derived tumor xenograft colon tumors in NOD/SCID mice in vivo

Figure 6 LacsRNA enhances anticancer activity of PD1 in B16F10 tumor-bearing C57 mice

Conclusion

Herein, a general method through a mild and simple chemistry was established to convert therapeutic miRNA into a stable and bioavailable IacsRNA. Driven by aurophilicity, IacsRNA self-assembled into a spherical nanostructure with the optimized anti-hydrolysis stability and low macrophage uptakes in comparison to conventional miRNA. As a consequence, IacsRNA presented the increased half-life period in circulation and accumulation at tumor sites in comparison to normal miRNA. More importantly, Iacs-miR-30c showed no toxicity of viscera and sanguis system in the 5-time injection dosage of the treatment. Expectedly, Iacs-miR-30c potently suppressed the Wnt signaling pathway in vitro and in vivo, and effectively sensitized both potency of 5-Fu in PDX model of colon cancer and Anti-PD1 in B16F10 homograft model of melanoma

Reference

Li L, He W, You W, Yan J, Liu W. Turing miRNA into infinite coordination supermolecule: a general and enabling nanoengineering strategy for resurrecting nuclear acid therapeutics. J Nanobiotechnology. 2022 Jan 4;20(1):10.