Transcriptomics is the study of all RNAs transcribed by a specific cell, tissue or individual at a specific time and state. Transcriptome sequencing uses the high-throughput sequencing methods to study specific cells, tissues or individuals at a specific time and state. All mRNAs transcribed under different functional states are used to reveal the differences in gene expression and structure under different functional states, and to clarify the molecular mechanism.
Journal of Nanobiotechnology Impact factor: 10.435 Published date: 2022 Published by: The Second Affiliated Hospital of Xi'an Jiaotong University
Nucleic acids exist in cell nucleus and mitochondria, they are important compounds in organism, they have important functions of storing and transmitting genetic information, and they are the basis material of genes. The clinical application of therapeutic nucleic acid, especially in the treatment of tumor, has broad prospects for development. However, due to its inherent weaknesses, poor system stability, fast clearance, low membrane permeability and the lack of targeting capabilities. Engineering small nucleic acids into carrier-free nanomedicines with structural stability and disease targeting may be a feasible way to overcome the nucleic acid medicine barrier. The second affiliated hospital of the Xi'an Jiaotong University is committed to this area of research, and has a number of research results in this field.
Herein, a general method through a mild and simple chemistry was established to convert therapeutic miRNA into a stable and bioavailable IacsRNA. Driven by aurophilicity, IacsRNA self-assembled into a spherical nanostructure with the optimized anti-hydrolysis stability and low macrophage uptakes in comparison to conventional miRNA. As a consequence, IacsRNA presented the increased half-life period in circulation and accumulation at tumor sites in comparison to normal miRNA. More importantly, Iacs-miR-30c showed no toxicity of viscera and sanguis system in the 5-time injection dosage of the treatment. Expectedly, Iacs-miR-30c potently suppressed the Wnt signaling pathway in vitro and in vivo, and effectively sensitized both potency of 5-Fu in PDX model of colon cancer and Anti-PD1 in B16F10 homograft model of melanoma
Li L, He W, You W, Yan J, Liu W. Turing miRNA into infinite coordination supermolecule: a general and enabling nanoengineering strategy for resurrecting nuclear acid therapeutics. J Nanobiotechnology. 2022 Jan 4;20(1):10.
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