Quantitative Analysis of Anthocyanins

Anthocyanins, are a class of water-soluble natural pigments that are widely found in plants. Most of the main color substances in fruits, vegetables and flowers are related to it. Absolute quantification of anthocyanins, analysis of quercetin, rutin, mallow pigment, etc.absolute qualitative and quantitative , and the analysis results are true and reliable. The relative quantification of anthocyanins can simultaneously conduct quantitative analysis and detection of hundreds of anthocyanins in plants, such as rutin, cyanidin, coumarin, etc.

Application Fields

Genetic breeding

Plant flower

Food industry

Identification and analysis

The mechanism of fruit coloring

Biosynthetic regulatory mechanisms

Bioactive functions (antioxidant, anti-inflammatory, anti-tumor)

Sample Requirements

Solid 2g
Sample type: plant tissue, soil etc.
Recommended to repeat more than 6 times

Liquid/solid samples, dry ice shipping; large pieces need to be cut into 1cm

Case Analysis

Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of arabidopsis carbon metabolism

Journal: Nature Communications Impact factor: 12.121 Published date: May, 2020 Published by: Michigan State University, the United States

Research Background

Anthocyanin pigments furnish a powerful visual output of the stress and metabolic status of Arabidopsis thaliana plants. Essential for pigment accumulation is TRANSPARENT TESTA19(TT19), a glutathione S-transferase proposed to bind and stabilize anthocyanins, participating in their vacuolar sequestration, a function conserved across the flowering plants.

Research Purpose

Identification of genetic suppressors that result in anthocyanin accumulation in the absence of TT19.

Research Result

mutations in RDR6, SGS3, or DCL4 suppress the anthocyanin defect of tt19 by pushing carbon towards flavonoid biosynthesis.

Figure 1 Molecular characterization of the tt19-8 phenotypic suppressors S1-S8 and corresponding anthocyanin accumulation

Figure 2 Over-expression of PAP1 is not sufficient to suppress the anthocyanin deficiency in tt19-8.

Figure 3 Flavonoids from COL-0 and Arabidopsis mutant plantlet grown in AIC. Use LC-MS/MS to compare a set of real known

Conclusion

Mutations in RDR6, SGS3, or DCL4 suppress the anthocyanin defect of tt19 by pushing carbon towards flavonoid biosynthesis. This effect is not unique to tt19 and extends to at least one other anthocyanin pathway gene mutant. This synergy between mutations in components of the RDR6-SGS3-DCL4 siRNA system and the flavonoid pathway reveals genetic/epigenetic mechanisms regulating metabolic fluxes.

Reference

Jiang Nan,Gutierrez-Diaz Aimer,Mukundi Eric et al. Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of Arabidopsis carbon metabolism.[J] .Nat Commun, 2020, 11: 2456