Quantitative Analysis of Carbohydrates

The chemical nature of sugars is polyhydroxy aldehyde or polyhydroxy ketone and its derivatives, which can be divided into monosaccharides, disaccharides and polysaccharides. The main functions of sugar in the human body: (1) As an energy substance, under normal circumstances, 70% of the energy required by the human body comes from the oxidation of sugar. (2) As structural components, glycoproteins and glycolipids are important components of cell membranes, and proteoglycans are structural components of connective tissues such as cartilage and bone. (3) Participate in the formation of biologically active substances, including sugars in nucleic acids, plasma proteins with transport functions, and antibodies with immune functions. Most membrane proteins recognized and transported are glycoproteins, as are many enzymes and hormones. (4) As a carbon source for the synthesis of other biomolecules, sugar can be used to synthesize substances such as lipids and amino acids.

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Sample Requirements

• Solid 2g
• Cells ≥ 5×10⁶
• Liquid 500μl
• Recommended to repeat more than 6 times

• Sample type: Animal and plant tissue, cells, blood, etc.

• Liquid/solid samples, dry ice shipping; large pieces need to be cut into 1cm pieces

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Case Analysis

Metabolic Profiles of Moso Bamboo in Response to Drought Stress in a Field Investigation

Journal: Science of the Total Environment       Impact factor: 6.551 Published date: June, 2020       Published by: Chinese Academy of Forestry

Research Background

n increasing number of moso bamboo habitats are suffering severe drought events. The improvement in our understanding of the mechanisms of drought-resistance in moso bamboo benefits their genetic improvement and maintenance of forest sustainability.

Research Method

Here, we investigated the metabolic changes across the annual growth cycle of moso bamboo in the field under drought stress using liquid chromatography coupled to mass spectrometry (LC-MS) based on untargeted metabolomic profiling

Figure 1 Changes of metabolite under drought stress

Figure 2 Bubble plots of D3 vs CK3, D6 vs K6, D9 vs CK9 showing enrichment of metabolites in affected pathways

Conclusion

The metabolic information provides new insights into the biochemical pathway underlying stress tolerance in bamboo plant.

Reference

Tong Ran,Zhou Benzhi,Cao Yonghui et al. Metabolic profiles of moso bamboo in response to drought stress in a field investigation.[J] .Sci. Total Environ., 2020, 720: 137722