Quantitative Analysis of Arachidonic Acids

Targeted metabolomics

Metabolic Flux Analysis

Quantitative Analysis of Energy Metabolism

Quantitative Analysis of Short-Chain Fatty Acids

Quantitative Analysis of Fatty Acids

Quantitative Analysis of Bile Acids

Quantitative Analysis of Trimethylamine Oxide and Related Metabolites

Quantitative Analysis of Amino Acids

Quantitative Analysis of Neurotransmitters

Quantitative Analysis of Organic Acids

Quantitative Analysis of Flavonoids

Quantitative Analysis of Carbohydrates

Quantitative Analysis of Plant Hormones

Quantitative Analysis of Carotenoids

Quantitative Analysis of Tannins

Quantitative Analysis of Phenolic Acids

Quantitative Analysis of Anthocyanins

Quantitative Analysis of Vitamins

Quantitative Analysis of Arachidonic Acids

Quantitative Analysis of Arachidonic Acids

Arachidonic acid is an 2-carbon unsaturated fatty acid. Under the action of inflammatory stimuli and mediators, it is metabolized by cyclooxygenase and lipid oxygenase pathways to generate various metabolites, mainly has prostaglandins (PG) and Leukotriene (LT). Inflammation stimulates arachidonic acid and releases its metabolites, led to inflammatory reactions such as fever, pain, vasodilation, increased permeability, and leukocyte exudation.


Application Fields

Research on the mechanism of disease

Disease marker

Evaluation of drugs


Sample Requirements


Case Analysis

Change of Tear Lipid Mediators in a Post-trabeculectomy Cohort

Journal: Ocular Surface       Impact factor: 12.33 Published date: July, 2020       Published by: National University of Singapore

Research Background

Trabeculectomy surgery could affect ocular surface disease (OSD) in several ways, through cessation of long term glaucoma eyedrops, exposure to operative mitomycin C and post-operative eyedrops including corticosteroids and aminoglycosides and reduction in eyelid hygiene measures. Previously we showed the relevance of tear lipid mediators (also referred oxylipins) in OSD. Here, we aim to evaluate changes of these lipids in a post-trabeculectomy cohort.

Research Method

Patients undergoing trabeculectomy were prospectively evaluated and had tear collected using Schirmer’s strips, preoperatively and postoperatively at 0.5, 1.0 and 3.0 years. Lipid mediators were analyzed using liquid chromatography mass spectrometry.

Research Result

The normalized concentrations of 40 lipid mediators were between 0.1-8.0 ng/mL, whereas docosahexaenoic acid (DHA), Arachidonic acid (AA) and eicosapentaenoic acid (EPA) ranged up to a few hundred ng/mL. The concentrations of lipid mediators, except DHA, EPA, and thromboxane (TXB1), showed reduction after surgery.

Conclusion

In this 3-years study, trabeculectomy reduced the tear level of proinflammatory lipid mediators.

Reference

Ambaw Yohannes Abere,Wong Tina,Chong Rachel et al. Change of tear lipid mediators in a post-trabeculectomy cohort.[J] .Ocul Surf, 2020

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