Plant Metabolomics Full Solutions

Application Fields

Research on abiotic environment relationships

Unsuitable environments create stress conditions, such as extreme high or low temperature, salinization and drought, which plants sense and respond to in order to survive.

Plants and microbes

Plants will produce their own immune response after being infected by pathogenic bacteria, and metabolites play a very important role in this immune response.

Application in phenotypic identification

Most genetically modified or knockout individuals lack significant phenotypic changes and can be distinguished by their metabolite content

Metabolic pathways and functional genomic studies

Unravel a range of substrates in metabolic pathways

Applications in medicinal plant research

Picking, processing of medicinal materials, quality control and monitoring of medicine metabolism in the body, development of medicinal plant species resources, etc.

Associated with human health research

Chemical structures, chemical synthesis pathways, bioavailability evaluations, and potential targets of plant active ingredients.

Metabolomics Technology

Untargeted Metabolomics

Qualitative and quantitative analysis of all small molecule metabolites in a biological system is an unbiased metabolomics analysis method.

Global Untargeted Metabolomics

Compared with ordinary untargeted metabolomics, the second generation of untargeted metabolomics has achieved a great improvement in the accuracy and accuracy of detecting substance content.

Lipidomics

Compare the changes of lipid metabolism network in different physiological states, and then identify the key lipid biomarkers in metabolic regulation, and finally reveal the mechanism of lipids in life activities.

Targeted Metabolomics

Quantitative analysis of fatty acids, short-chain fatty acids, bile acids, neurotransmitters, amino acids, organic acids, TMAO and related metabolites.

Case Analysis

HEAT INDUCIBLE LIPASE1 Remodels Chloroplastic Monogalactosyldiacylglycerol by Liberating α-Linolenic Acid in Arabidopsis Leaves under Heat Stress

Journal: Plant Cell Impact factor: 9.618 Published date: 2018 Published by: Kanagawa Sustainable Resources Science Center, Japan

Research Background

Under heat stress, polyunsaturated acyl groups, such as α-linolenate (18:3) and hexadecatrienoate (16:3), are removed from chloroplastic glycerolipids in various plant species.

Research Design

Here, we showed that a lipase designated HEAT INDUCIBLE LIPASE1 (HIL1) induces the catabolism of monogalactosyldiacylglycerol (MGDG) under heat stress in Arabidopsis thaliana leaves. Using thermotolerance tests, a T-DNA insertion mutant with disrupted HIL1 was shown to have a heat stress-sensitive phenotype. Lipidomic analysis indicated that the decrease of 34:6-MGDG under heat stress was partially impaired in the hil1 mutant. Concomitantly, the heat-induced increment of 54:9-triacylglycerol in the hil1 mutant was 18% lower than that in the wild-type plants. Recombinant HIL1 protein digested MGDG to produce 18:3-free fatty acid (18:3-FFA), but not 18:0- and 16:0-FFAs. A transient assay using fluorescent fusion proteins confirmed chloroplastic localization of HIL1. Transcriptome coexpression network analysis using public databases demonstrated that the HIL1 homolog expression levels in various terrestrial plants are tightly associated with chloroplastic heat stress responses. Thus, HIL1 encodes a chloroplastic MGDG lipase that releases 18:3-FFA in the first committed step of 34:6 (18:3/16:3)-containing galactolipid turnover, suggesting that HIL1 has an important role in the lipid remodeling process induced by heat stress in plants.

Figure 1 HIL1 homologous genes are evolutionarily conserved in plant species and HIL1-like proteins have an indispensable role in terrestrial plants

Figure 2 Gene expression analysis of HIL1 found that it is closely related to CLPB3 and DGD1, and is present in soybean, tomato and other plants

Figure 3 Recombinant HIL1 proteolytically hydrolyzes MGDG to release 18:3

Reference

Y. Higashia et al., HEAT INDUCIBLE LIPASE1 Remodels Chloroplastic Monogalactosyldiacylglycerol by Liberating α-Linolenic Acid in Arabidopsis Leaves under Heat Stress. The Plant Cell, Vol. 30: 1887–1905, August 2018