10× Genomics Single Cell Transcriptome

10X Genomics offers the Chromium single-cell solution that captures molecular readouts of cellular activity from multiple perspectives. Based on Next GEM technology, the core of the platform is the ability to generate thousands of single-cell microdroplets, each containing an identifiable barcode for downstream analysis. 10X Genomics Chromium system uses advanced microfluidics technology to generate and barcode single-cell microdroplets in minutes, which can then be followed up with library preparation and seamlessly integrated with the the illumina platform is seamlessly integrated. 10x Genomics single-cell transcriptome sequencing effectively solves the challenge of cell heterogeneity, which cannot be deciphered by tissue samples, and helps to discover new cell types; and based on a large amount of single-cell gene expression data, it can provide cell expression signature clustering, analysis of subpopulation expression signature, and marker screening, which is an efficient technology for single-cell gene expression level detection.

Case Sharing

Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq

Published Journal：Cell Discovery Impact Factor：33.5 Published：2022

Research background

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is currently an effective treatment for hematologic malignancies, but post-transplantation comorbidity graft-versus-host disease (GVHD) is one of the major causes of death in post-transplant patients. GVHD is one of the major causes of death in post-transplant patients. Granulocyte colony-stimulating factor (G-CSF) is a widely used clinical hematopoietic stem cell mobilizer that reduces the incidence of acute GVHD through immunomodulatory effects without affecting the graft-versus-host disease effect. Furthermore, studies have shown that G-CSF mobilized bone marrow (G-BM) transplantation has comparable implantation outcomes and lower risk of GVHD than G-CSF mobilized peripheral blood stem cell transplantation, suggesting that G-BM has unique immunomodulatory properties. However, the mechanisms by which G-CSF affects human bone marrow hematopoietic differentiation and induces immune tolerance have not been precisely elucidated to date.

Technology Roadmap

Partial results

Research findings

This study analyzed the single-cell transcriptome profile of human bone marrow before and after mobilization with G-CSF, revealing the cellular hierarchy of G-CSF-induced myeloid skewing in hematopoietic differentiation and providing a detailed understanding of the molecular features and potential mechanisms by which G-CSF induces T/NK cell immune hypo-responsiveness through direct and indirect pathways. These findings provide important clues for optimizing HSCT strategies for treating hematological malignancies and preventing GVHD in clinical settings.

Reference

You G, Zhang M, Bian Z, et al. Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq [J]. Cell Discov. 2022, 8(1):59.