DIA Proteome Analysis Technology

Data-Independent Acquisition (DIA) has gradually emerged. Compared with the DDA data acquisition mode, the limited peptide ions (usually TOP20) with higher response values are collected in the primary mass spectrometer in each time window and enter the secondary mass spectrometer for fragmentation analysis. DIA divides the entire full scan range of mass spectrometry into several windows, and selects, fragments and detects all ions in each window at high speed and cyclically, so as to obtain all fragmentation information of all ions in the sample without omission and difference.

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Research Process

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Analysis Result

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Case Analysis

Complement Pathway Changes at Age 12 are Associated with Psychotic Experiences at Age 18 in a Longitudinal Population-Based Study: Evidence for a Role of Stress

Journal: Molecular Psychiatry       Impact factor: 11.973 Published date: 2019       Published by: Royal College of Surgeons of Ireland

Research Background

The complement cascade is a major component of the immune defence against infection, and there is increasing evidence for a role of dysregulated complement in major psychiatric disorders.

Research Purpose

To further demonstrate the relationship between them, the researchers performed DIA proteomic testing on blood samples from the UK avon longitudinal study of parents and children (ALSPAC) cohort, focusing on 29 complement cascade-related protein expression levels.

Research Result

The expression levels of 6 proteins were associated with mental disorders. Using a rat model of chronic stress, the results of the study further showed that chronic stress may cause abnormalities in the complement pathway, which is significantly correlated with mental disorders.

Reference

Föcking, Melanie, Sabherwal S , Cates H M , et al. Complement pathway changes at age 12 are associated with psychotic experiences at age 18 in a longitudinal population-based study: evidence for a role of stress[J]. Molecular Psychiatry, 2019